Day 1 | Day 2 | Day 3
Sponsors

Posters
Day 2: TP22
Automated LC/MS Analysis of Biomolecules Using ProMass^TM

Mark E. Hail, Jeffrey L. Whitney, and David J. Detlefsen
Novatia, LLC, Princeton, NJ 08540

The recent increase in genomic and proteomic discovery has increased the need for high-throughput automated tools for biomolecule characterization. The flood of new protein targets will ultimately demand more efficient tools for evaluation of expressed proteins for drug discovery and structural biology studies. ProMass is an automated biomolecule deconvolution and reporting program that is used to process LC/ESI/MS data or single ESI mass spectra to derive molecular mass information. We have recently integrated ProMass with the ThermoFinnigan Xcalibur data system to create a version of ProMass that is known as ProMass Xcali. ProMass Xcali processes entire Xcalibur sample sequences, deconvolutes mass spectra from LC/MS data, and produces web-based reports. ProMass Xcali uses a novel cross-platform deconvolution algorithm known as ZNova. ZNova incorporates a unique charge-state scoring method that assigns the charge states of all signals in the ESI mass spectra and transforms the input ESI mass spectra to produce zero-charge mass spectra (i.e., molecular mass information). ZNova incorporates signal processing techniques and unique logic that allow application to low charge state spectra and data of low signal-to-noise ratio. As a result, ZNova can be used to process data from a wide variety of biomolecules including large proteins, oligonucleotides, peptides, etc. In this poster, an overview of the ProMass Xcali / ZNova system will be presented along with selected applications which highlight its utility in a high-throughput environment.

Return to Day 2