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Day 2: TP30
Advantages and Limitations of Increased Mass Resolution for Quantitative SRM Analysis on a Triple Stage Quadrupole Mass Spectrometer

Hans Schweingruber, Jean-Jacques Dunyach, Terry N. Olney, Gary Paul, Dennis Taylor, Mark Churchill, Ma'an Amad, Witold Winnik, Alan E. Schoen, Clay S. Campbell, and Iain Mylchreest
ThermoFinnigan Corporation

The benefits of high resolution for quantitative selected reaction monitoring (SRM) analysis using the new TSQ Quantum triple quadrupole mass spectrometer are discussed. Examples will be shown where the high resolution capability of the TSQ Quantum results in much-improved signal to noise ratios (S/N) for analytes relative to those achieved at unit resolution as a consequence of the removal of isobaric impurities present in the matrix by high resolution. This improvement in S/N can result in a lower LOQ, increased dynamic range and improved accuracy and precision for lower analyte concentration measurements. The increased selectivity in SRM afforded by high resolution (Q1 0.1 Da) comes with only a small decrease in analyte signal of typically x2-3 compared to unit resolution operation (Q1 0.7 Da). Hence with the corresponding decrease in background noise achieved by high resolution, LOQ values as low as 10 fg on-column can be observed on the TSQ Quantum in both unit and high resolution modes.

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