CPSA Digest 2002

Proceedings -Wednesday, October 9, 2002

WeOD3

New Strategies for Rapid Analytical Method Development in Support of Lead Optimization

Background
In recent years, the goal of discovery analysis has shifted from a sole pursuit of high throughput techniques toward performing high quality analyses. Under this new model, the biggest challenges to analysts are to minimize assay time by utilizing newer technologies and also to perform rapid analytical method development. The goals from these approaches are to solve more specific problems and also to maximize the information obtained from each single run.

Premise
Several new multidisciplinary approaches in separation sciences have been successfully implemented within the Small Molecule Analytical Group at Amgen. Toward the goal of accelerating the overall method development process, the following activities have been applied:

1. Implementing structure/recognition-based parallel method development using supercritical fluid chromatography (SFC) and capillary electrophoresis (CE) in complement to liquid chromatography
2. Applying column-coupling or switching to maximize the selectivity for simultaneous achiral and chiral separation
3. Using SFC-MS with sample-pooling to develop multiple chiral separation methods in a short time frame
4. Performing post-column addition to characterize very polar biological compounds

Applications for the following techniques were shared during this presentation:

• High throughput LC-MS
• LC-TOF-MS
• Preparative LC-MS
• Chiral separations
• SFC-MS
• High throughput preparative SFC with UV-MS
• High throughput affinity LC-MS

References and/or Links
Poster at CPSA: High throughput LogP measurement using parallel LC-UV-MS and sample pooling, Yining Zhao and Ed Maliski, Amgen Inc.

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