CPSA Digest 2003

Day 1

Vendor Session - Proteomics Technologies & Applications

Proteome Analysis of Human Plasma with the ProteomeLab™ PF-2D System

Traditionally profiling has been accomplished by two-dimensional gel electrophoresis. Although two-dimensional polyacrylamide gel electrophoresis has successfully resolved proteomes, it is labor intensive, time consuming, and, at best, semi-quantative. This poster describes an alternative approach to two-dimensional gel electrophoresis for the discovery phase of proteomics. A complete system, the ProteomeLab™ PF-2D has been developed with integrated hardware, software, robotics and chemistry. This system uses two-dimensional liquid chromatography. The first dimension is chromatofocusing, which separates based on the isoelectric point (pI) of proteins. Fractions are collected in the first dimension and subsequently, separated by a second dimension of reversed phase chromatography, which separates based on hydrophobicity. The differential fractionation of "intact" proteins in an all liquid state is achieved. Proprietary ProteomeView™ software suite will quickly generate protein maps and produce a differential display of the protein maps of interest.

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