Increased Throughput and Separation in Nano-HPLC-MS Using a
Dual-Gradient Nano-HPLC System and Monolithic Columns
Remco van Soest
LC Packings/Dionex
Capillary and Nano HPLC are used in proteomics because of the tremendous
increase in sensitivity compared with conventional HPLC when coupled
with mass spectrometry. In this presentation two new products will be
introduced that increase both sample throughput and separation power:
the Dual-Gradient UltiMate system and our Monolithic columns for
peptide/protein separations. The Dual-Gradient system can be used
either in parallel mode to increase throughput or can be set up for
2-dimensional HPLC to obtain better separations for samples containing
thousands of peptides. Applications showing increased throughput and
separation capacity in proteomics will be given.