CPSA Digest 2003

Day 1

Vendor Session - Proteomics Technologies & Applications

Protein Expression Profiling: Current Trends and Future Horizons

Protein expression and quantitation is attracting considerable interest in the life science community and is developing as one of the major challenges in defining mechanisms of action in biological systems. Mass spectrometry, in its variety of forms, holds significant promise for determining relative levels of protein and peptides in complex mixtures. In this short presentation, chemical and instrumental methods will be discussed that allow quantitative analysis of peptides from complex mixtures. Reagents like ICAT and TMPP (2,4,6-trimethoxy phenol phosphonium acetic acid N-hydroxysuccinimide ester) will be compared and contrasted. Additionally, appropriate analytical methods and software that allow peptides to be analyzed with greater specificity and in some cases greater sensitivity will be discussed. Software that then allows for the extension of peptide quantitative measurements to protein expression will also be presented.

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