CPSA Digest 2003

Day 2

Vendor Session - Drug Discovery Technologies & Applications

Automated Method Development for Solid Phase Extraction in an Online
SPE/LC/MS System

A scientific approach for developing SPE methods in a fully automated SPE/LC/MS system has been introduced. Key in developing SPE methods is to find the best suitable sorbent/solvents combination that provides the highest extraction of the compounds of interest and the best clean-up from the matrix components in the sample. There are endless combinations to try, therefore traditionally this has been a lengthy and complex process. Secondly, the results traditionally represented only the recovery on the extraction cartridge tested. In the proposed setup two SPE cartridges will be placed in series and are eluted individually. The Spark Holland SymbiosisTM system not only will perform fully automated each of the chosen combination of SPE protocols; it will also present results on recovery, breakthrough and tubing adsorption. The obtained additional information and the automated extraction has lead to a significant reduction in the time spend on Method Development.

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