CPSA Digest 2003

Day 2

Vendor Session - Drug Discovery Technologies & Applications

Parallel Liquid Chromatography for High Throughput ADMET Profiling

The Nanostream Veloce system–which includes an instrument, software, and replaceable microfluidic cartridges–incorporates pressure-driven flow to achieve chromatograms comparable to conventional HPLC instrumentation while offering a dramatic increase in sample analysis capacity. The system enables parallel chromatographic separations and simultaneous, real-time UV detection for rapid determination of ADMET properties. Each Nanostream Brio™ cartridge, made of polymeric materials, incorporates twenty-four columns packed with standard (C-18) stationary phase material to achieve reverse-phase separations. Mixing and distribution of the mobile phase to each of the 24 columns is precisely controlled in each cartridge ensuring excellent reproducibility within a single column and between columns. The system provides an ideal platform to accelerate assessment of physicochemical properties for a large number of compounds. This talk will focus on how this system has been used to accelerate the profiling of ADMET properties, like solubility, CHI and Log P. The data presented will show a 24-fold increase in sample analysis capacity, which allows for rapid generation of standard curves and simultaneous analysis of multiple replicates in a single run. This system may also be used to accelerate pharmacokinetic and in-vivo/in-vitro metabolite stability assays.

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