CPSA Digest 2003

Day 3

Vendor/Exhibitor Session

Strategies for Metabolite Identification Using a Hybrid Quadrupole Linear Ion Trap Mass Spectrometer

4000 Q Trap™ System is the next generation hybrid linear ion trap/triple quadrupole mass spectrometer. It provides full scan sensitivity and MS-cubed capabilities (full scan MS, MS/MS and MS3) as with the traditional 3D trap but maintains the high sensitivity triple quadrupole performance (single MS scan modes Q1/Q3, neutral loss, precursor ion and MRM sensitivity) of the leading API 4000™ System. These capabilities can be combined in one experiment using AnalystŪ and IDA software to maximize the information gained in a single run. Additional features include enhanced resolution, better mass accuracy and a LINAC® collision cell for multi-component analyses. Overall, the 4000 Q Trap™ System is a unique solution to identify, confirm and quantify compounds.

Application Example
Phase I metabolism study of Remikiren in rat hepatocytes. The ability to trigger confirmation MS/MS scans off MRM transitions so that searching for expected metabolites is now a lot easier. For example, 26 MRM transitions can be monitored to detect for six common biotransformations. Overall, the MRM method builder allowed identification and confirmation of 14 metabolites; compared with nine metabolites identified by precursor ion and four metabolites by enhanced MS scan.

Contact Information
Applied Biosystems/MDS Sciex
850 Lincoln Centre Drive
Foster City, CA 94404
800-327-3002
650-638-5800
650-638-5884 Fax
www.appliedbiosystems.com

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