CPSA Digest 2003

Day 3

Vendor/Exhibitor Session

Accurate Mass Measurement on the Finnigan TSQ Quantum Ultra AM

The new Finnigan TSQ Quantum Ultra AM has the capability of routine accurate mass (AM) measurements in both SIM MS and MS/MS modes. Its diligently engineered analyzer control circuitry allows full advantage of the high-resolution HyperQuad mass analyzers that are at the heart of every Finnigan TSQ Quantum. This additional innovation imparts accurate mass measurement capabilities to this triple quad MS. Ease of use and a greater dynamic range than those of instruments based on Q-ToF technology are some of the other significant features of this platform. For example, AM measurements from the Quantum Ultra AM are sufficient to obtain elemental compositions of metabolites, while AutoSIM allows data-dependent accurate mass in either MS or MS/MS modes.

The accurate mass measurement option on the triple quad can be used in two ways: internal lock mass or external lock mass. Using an INTERNAL lock, the lock masses are present in the spectrum while the analyte is being measured. Accuracy is very good (milli-Daltons) but when a TIC is performed, a raised baseline results due to the infusion of PEGs for the internal lock.

It is preferable to use an EXTERNAL lock. In this example, the lock masses have already been infused and the necessary corrections have been measured. The corrections are frozen and continue to be applied to the spectrum until the External lock is turned off. Note that the baseline is near zero intensity since there is no infusion of lock masses.

Some examples of accurate mass measurement include:

• In vitro metabolism to uniquely identify specific elemental compositions
• In Vitro Data-dependent accurate mass MS/MS
• In Vivo Metabolism

Contact Linformation
Thermo Electron Corporation
265 Davidson Avenue
Suite 101
Somerset, NJ 08873
800-631-5693
732-627-0220
732-627-0260 Fax
www.thermo.com/com/cda/product/detail/1,1055,10558,00.html

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