CPSA Digest 2004

Day 1 - Tuesday, October 19, 2004

Vendor Session - Biomarker Identification with the NanoLC System

• NanoLC Proteomics System
  • Direct pumping: no flow splitting
  • Precise, splitless flow control to 20 nl/min
  • Microfluidic flow control
    • Flow meters in each mobile phase measure the actual flow rate
    • Active Flow Control uses feedback to control a rapid, electronically adjusted variable pressure source
    • Flow rate is maintained regardless of system back pressure or viscosity
  • Rapid flow control at nanoscale flow rates: 5x flow rate change achieved in <1 sec
  • Improved S/N with reduced flow rate: >6x more signal using ~4x less sample
  • Dynamic flow control: able to adjust flow rate during an experiment and increase acquisition time when required
  • Peak parking capability
    • Repeated flow rate reductions (400-50 nL/min)
    • Gradient profile resumed after parking
    • Parking flow can be set at any rate
    • Triggered within software or by external trigger
  • Phosphopeptide identification with Peak Parking
    • Mixture of a and b casein with BSA tryptic digests
    • Q-Trap triple quadrupole with linear trap
    • Neutral loss (49 amu) survey scan to identify phosphopeptides
    • Peak parking activated by MS software when neutral loss detected
  • 1-D and automated 2-D versions
  • Enhances MS detection sensitivity
  • Integrated with Xcalibur and Analyst 1.4.1

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