CPSA Digest 2004

Day 1 - Tuesday, October 19, 2004

Vendor Session - MALDI Imaging Software for QSTAR® XL System

• A novel software package used to acquire and process 2- and 3-dimensional images using an oMALDI™ QSTAR® System
  • Assists researchers in learning about drug metabolism and distribution of drugs throughout tissue
  • Permits ion images to be created from analysis of tissue imprinted membranes
  • Advantages of MALDI-MS Imaging
    • Offers spatial relationship information of autoradiography/ fluorescence spectroscopy and compound specificity of homogenization LC/MS/MS approach
    • Applies to a wide range of tissue samples
    • No hardware modification required
    • Can be performed in the early drug discovery cycle
  • Surface of Porcine Epidermal Tissue
    • Treated tissue blotted onto cellulose membrane precoated in a-CHCA
    • MALDI Image of the distribution of the protonated molecule [M+H]+ at m/z 532 of Ketoconazole on the surface of porcine skin acquired after topical application
    • Examination of individual image pixels and their corresponding mass spectra permits the construction of a quantitative profile of ketoconazole in skin.
    • Comparison with a histological image of the tissue implies drug permeation up to and including the dermal skin layer

Superimposed MALDI MS and Histological Images

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