CPSA Digest 2004

Day 2 - Wednesday, October 20, 2004

Vendor Session - Combined Tissue Distribution, Tissue Quantitation, and Metabolite Profiling using QWBA, LC/FSD, LC/MS/MS

• Discovery QWBA + LC/FSD/MS/MS:
  • Provides detail metabolic profile at the organ/tissue level
  • Advantages
    • Minimum animal use: 2-4
    • Quick: From dose to preliminary metabolic profile can be within 2 weeks
    • QWBA: radioactivity distribution at the organ/tissue level
    • LC/FSD: metabolic profiles at the organ/tissue level
    • LC/MS/MS: metabolite identification/elucidation
    • Frozen carcass can be further section at <1mm for MALDI MS profiling, and antibody staining for toxicity studies.
  • An excellent tool for mechanism of action, active metabolites, organ toxicity studies

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