CPSA Digest 2004

Day 2 - Wednesday, October 20, 2004

Vendor Session - Application of Nanobore LC-MS to the Analysis of Small Drug Molecules

• Nanospray System Configuration
  • A standard 6-port injection scheme (1 µL volume) is used with in-line filtration (1 µm porosity) to preserve column integrity
  • ESI high voltage is applied pre-column directly to the mobile phase through a platinum wire micro-electrode
  • Pre-column volume is kept to a minimum (= 1 µL) to minimize the gradient delay
• Peak Parking Gradient
  • The conventional gradient at 250 nL/min went from 10% B to 90% B over a 3.5 minute period of time
  • The gradient delay between pump and column was approximately 3 minutes
• Nano-Flow Peak Parking
  • Peak parking gradient
  • 250 nL/min to 150 nL/min
  • 1 µL injection
  • 10 pg on-column

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