Short Courses

Tuesday, October 17

• Method Development for LC/MS: Traditional Approaches and Emerging Trends
  
  

  This practical course on HPLC method development for LC/MS covers the specifics of how sample preparation and HPLC methods are developed for interfacing to ESI and APCI. The course discusses the importance of HPLC when interfaced with mass spectrometry including the HPLC separation of undetected matrix components, isomers and labile metabolites. The development of HPLC methods for LC/MS for use in qualitative and quantitative analysis throughout drug discovery and drug development are presented. The most advantageous column chemistry for each LC/MS application are thoroughly discussed including, reverse phase, normal phase, ion-exchange, HILIC, monolithic and polar embedded phases. The latest technologies such as UPLC, micro-SPE and on-line SPE will also be discussed. Orthogonal approaches to extraction and chromatography to achieve optimized LC/MS methods will be presented. Step-wise method development tutorials of developing methods for specific compound classes based on the structure are included in the final summary.
  
  Instructors: Roger Hayes, Schering-Plough Research Institute; Shane Needham, Alturas Analytics