Short Courses

Monday, October 22
Bucks county Sheraton Hotel
Langhorne, PA

• Method Development for LC/MS: Traditional Approaches and Emerging Trends
  
  

  This practical course on HPLC method development for LC/MS covers the specifics of how sample preparation and HPLC methods are developed for interfacing to ESI and APCI. The course discusses the importance of HPLC when interfaced with mass spectrometry including the HPLC separation of undetected matrix components, isomers and labile metabolites. The development of HPLC methods for LC/MS for use in qualitative and quantitative analysis throughout drug discovery and drug development are presented. The most advantageous column chemistry for each LC/MS application are thoroughly discussed including, reverse phase, normal phase, ion-exchange, HILIC, monolithic and polar embedded phases. The latest technologies such as UPLC, micro-SPE and on-line SPE will also be discussed. Orthogonal approaches to extraction and chromatography to achieve optimized LC/MS methods will be presented. Step-wise method development tutorials of developing methods for specific compound classes based on the structure are included in the final summary.
  
  Instructors: Roger Hayes, Schering-Plough Research Institute; Shane Needham, Alturas Analytics



• High Throughput Drug Discovery Support: LC/MS Strategies for Method Development, Profiling and Workflow Solutions
  
  

  Integrated HPLC and MS approaches for high throughput analysis have become standard practice in a pre-clinical drug discovery setting. Successful support strategies often provide a blend of high throughput methodologies with appropriate resources (tools and personnel). This course will target the “bread and butter” functions of discovery medicinal chemistry support. Real-world experiences with method development and analytical support will be discussed. Examples that feature ultra high throughput (library-based formats and individual sample processing), fast LC (theory & practice), generic gradient RP method strategies , scaling for HT purification, building in robustness into LC/MS set ups, details and perspectives on open-access success, fast physico-chemical profiling of compounds, and IT based workflow solutions.
  
  Instructors: Mark Hayward, Lundbeck Research USA; Ken Lewis, OpAns